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These animals were broadly representative of the New Zealand dairy population, where 18,854 of the 64,244 animals targeted for imputation had a sire directly represented in the sequence reference, and an additional 28,564 animals had at least one grandsire represented. Variants were called from sequence alignments using RTG Core21, yielding 109,517 variants in the 10Mbp target interval. These variants were then imputed into the phenotypic population using Beagle software (v4)22, applying a stepwise imputation strategy to integrate data across different genotyping platforms.

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